AIM: To determine the effects of substrate concentration variation on the activity of the enzyme pepsin. Also to determine the level of substrate concentration at which the enzyme pepsin functions most efficiently.

HYPOTHESIS: The percent of substrate concentration that the enzyme pepsin will function at its optimum will be 10%.

INDEPENDENT VARIABLE: The concentration of the substrate (egg albumin) used in the experiment.

DEPENDENT VARIABLE: The efficiency of the enzyme in acting and breaking down the protein in the egg albumin, i.e. how much egg albumin is dissolved.

CONTROL: The HCl solution without the enzyme pepsin.

EQUIPMENT:

☺46 large test tubes

☺1 beaker

☺2 thermometers

☺ Pepsin powder (2g)

☺ Distilled water

☺ Hydrochloric acid (HCl-1 molar)

☺ Egg albumin

☺ Test tube rack x4

☺ Data Logger + Light Probes

☺ Transformer

☺ Computer + Tainlab

☺ Light Globe

☺ Pipette

☺ Syringe

☺ Black Box Set-up (See Diagram 1)

☺ Bunsen

☺ Test tube holder

☺ Matches

☺ Retort stand

☺ Filter funnel

☺ Muslin...