I. Purpose- The purpose of this DNA experiment is to analyze and get a better understanding of restriction enzymes and how to isolate a particular gene to manipulate it. This experiment will introduce us to biotechnology by isolating and manipulating DNA. We will perform an analysis on Lambda DNA using techniques of genetic engineering with restriction enzymes. We will cut the Lambda DNA into different fragments using restriction enzymes and separate the DNA fragments using gel electrophoresis. This experiment will also allow us to get used to scientific instruments like the pipettes and gel box.

II. Materials-

4. 1.5 ìl reaction tubes Cups
3. small enzyme tubes (Hind III, Eco RI, Bam HI) Foam block
1. 1.5 ìl reaction tube with 1.0 ìl distilled water Eppendorf

Micro centrifuge tubes Micropipettes and tips

37oC incubator or water bath Buffer

Five ìl of DNA Dye

Incubator...