The summit of another bladder, the glands of which were in a beautifully clear condition, was irrigated with a few drops of a mixed solution of nitrate and phosphate of ammonia, each of one part to 437 of water.  After 2 hrs. some few of the glands were brownish.  After 8 hrs. almost all the oblong glands were brown and much more opaque than they were before; their primordial utricles were somewhat shrunk and contained a little aggregated granular matter.  The spherical glands were still white, but their utricles were broken up into three or four small hyaline spheres, with an irregularly contracted mass in the middle of the basal part.  These smaller spheres changed their forms in the course of a few hours and some of them disappeared.  By the next morning, after 23 hrs. 30 m., they had all disappeared, and the glands were brown; their utricles now formed a globular shrunken mass in the middle.  The utricles of the oblong glands had shrunk very little, but their contents were somewhat aggregated.  Lastly, the summit of a bladder which had been previously irrigated for 21 hrs. with a solution of one part of sugar to 218 of water without being affected, was treated with the above mixed solution; and after 8 hrs. 30 m. all the glands became brown, with their primordial utricles slightly shrunk.

Four summits were irrigated with a putrid infusion of raw [page 420] meat.  No change in the glands was observable for some hours, but after 24 hrs. most of them had become brownish, and more opaque and granular than they were before.  In these specimens, as in those irrigated with the salts of ammonia, the nuclei seemed to have increased both in size and solidity, but they were not measured.  Five summits were also irrigated with a fresh infusion of raw meat; three of these were not at all affected in 24 hrs., but the glands of the other two had perhaps become more granular.  One of the specimens which was not affected was then irrigated with the mixed solution of the nitrate and phosphate of ammonia, and after only 25 m. the glands contained from four or five to a dozen granules.  After six additional hours their primordial utricles were greatly shrunk.

The summit of a bladder was examined, and all the glands found colourless, with their primordial utricles not at all shrunk; yet many of the oblong glands contained granules just resolvable with No. 8 of Hartnack.  It was then irrigated with a few drops of a solution of one part of urea to 218 of water.  After 2 hrs. 25 m. the spherical glands were still colourless; whilst the oblong and two-armed ones were of a brownish tint, and their primordial utricles much shrunk, some containing distinctly visible granules.  After 9 hrs. some of the spherical glands were brownish, and the oblong glands were still more changed, but they contained fewer separate granules; their nuclei, on the other hand, appeared larger, as if they had absorbed the granules.  After 23 hrs. all the glands were brown, their primordial utricles greatly shrunk, and in many cases ruptured.