In a leaf which had been left for 22 hrs. in a solution of one part of the carbonate to 292 of water, some spheres of protoplasm (formed by the self-division of a bag-like mass) were gently pressed beneath a covering glass, and then examined under a high power. They were now distinctly divided by well-defined radiating fissures, or were broken up into separate fragments with sharp edges; and they were solid to the centre. In the larger broken spheres the central part was more opaque, darker-coloured, and less brittle than the exterior; the latter alone being in some cases penetrated by the fissures. In many of the spheres the line of separation between the outer and inner parts was tolerably well defined. The outer parts were of exactly the same very pale purple tint, as that of the last formed smaller spheres; and these latter did not include any darker central core.
From these several facts we may conclude that when vigorous dark-coloured leaves are subjected to the action of carbonate of [page 48] ammonia, the fluid within the cells of the tentacles often aggregates exteriorly into coherent viscid matter, forming a kind of bag. Small spheres sometimes appear within this bag, and the whole generally soon divides into two or more spheres, which repeatedly coalesce and redivide. After a longer or shorter time the granules in the colourless layer of protoplasm, which flows round the walls, are drawn to and unite with the larger spheres, or form small independent spheres; these latter being of a much paler colour, and more brittle than the first aggregated masses. After the granules of protoplasm have been thus attracted, the layer of flowing protoplasm can no longer be distinguished, though a current of limpid fluid still flows round the walls.
If a leaf is immersed in a very strong, almost concentrated, solution of carbonate of ammonia, the glands are instantly blackened, and they secrete copiously; but no movement of the tentacles ensues. Two leaves thus treated became after 1 hr. flaccid, and seemed killed; all the cells in their tentacles contained spheres of protoplasm, but these were small and discoloured. Two other leaves were placed in a solution not quite