but I at first thought that the solution might not have travelled down the cells of the tentacles, where the protoplasm was seen in active movement.  This view, however, I have no doubt, is erroneous, for a leaf which had been immersed for 3 hrs. in the quinine solution was then placed in a little solution of one part of carbonate of ammonia to 218 of water; and in 30 m. the glands and the upper cells of the tentacles became intensely black, with the protoplasm presenting a very unusual appearance; for it

* ‘Quarterly Journal of Microscopical Science,’ April 1874, p. 185. [page 202]

had become aggregated into reticulated dingy-coloured masses, having rounded and angular interspaces.  As I have never seen this effect produced by the carbonate of ammonia alone, it must be attributed to the previous action of the quinine.  These reticulated masses were watched for some time, but did not change their forms; so that the protoplasm no doubt had been killed by the combined action of the two salts, though exposed to them for only a short time.

Another leaf, after an immersion for 24 hrs. in the quinine solution, became somewhat flaccid, and the protoplasm in all the cells was aggregated.  Many of the aggregated masses were discoloured, and presented a granular appearance; they were spherical, or elongated, or still more commonly consisted of little curved chains of small globules.  None of these masses exhibited the least movement, and no doubt were all dead.

Half-minims of the solution were placed on the discs of six leaves; after 23 hrs. one had all its tentacles, two had a few, and the others none inflected; so that the discal glands, when irritated by this salt, do not transmit any strong motor impulse to the outer tentacles.  After 48 hrs. the glands on the discs of all six leaves were evidently much injured or quite killed.  It is clear that this salt is highly poisonous.*

Acetate of Quinine.—­Four leaves were immersed, each in thirty minims of a solution of one part to 437 of water.  The solution was tested with litmus paper, and was not acid.  After only 10 m. all four leaves were greatly, and after 6 hrs. immensely, inflected.  They were then left in water for 60 hrs., but never re-expanded; the glands were white, and the leaves evidently dead.  This salt is far more efficient than the sulphate in causing inflection, and, like that salt, is highly poisonous.

Nitrate of Quinine.—­Four leaves were immersed, each in thirty minims of a solution of one part to 437 of water.  After 6 hrs. there was hardly a trace of inflection; after 22 hrs. three of the leaves were moderately, and the fourth slightly inflected; so that this salt induces, though rather slowly, well-marked inflection.  These leaves, on being left in water for 48 hrs., almost

Binz found several years ago (as stated in ’The Journal of Anatomy and Phys.’  November 1872, p. 195) that quinia is an energetic poison to low vegetable and animal organisms.  Even one part added to 4000 parts of blood arrests the movements of the white corpuscles, which become “rounded and granular.”  In the tentacles of Drosera the aggregated masses of protoplasm, which appeared killed by the quinine, likewise presented a granular appearance.  A similar appearance is caused by very hot water. [page 203]