(7) After 4 hrs. 30 m. only thirty-five tentacles inflected or sub-inflected, and this small amount of inflection never increased.
Now for the seven corresponding leaves in water:—
(1) After 4 hrs. thirty-eight tentacles inflected; but after 7 hrs. these, with the exception of six, re-expanded.
(2) After 4 hrs. 20 m. twenty inflected; these after 9 hrs. partially re-expanded.
(3) After 4 hrs. five inflected, which began to re-expand after 7 hrs.
(4) After 24 hrs. one inflected.
(5), (6) and (7) Not at all affected, though observed for 24 hrs., excepting the short tentacles on the borders of the disc, which as usual formed a ring.
A comparison of the leaves in the solution, especially of the first five or even six on the list, with those in the water, after 1 hr. or after 4 hrs., and in a still more marked degree after 7 hrs. or 8 hrs., could not leave the least doubt that the solution had produced a great effect. This was shown not only by the vastly greater number of inflected tentacles, but by the degree or closeness of their inflection, and by that of their blades. Yet each gland on leaf No. 1 (which bore 255 glands, all of which, excepting five, were inflected in 30 m.) could not have received more than one-four-millionth of a grain (.0000162 mg.) of the salt. Again, each gland on leaf No. 3 (which bore 233 glands, all of which, except nine, were inflected in 2 hrs. 30 m.) could have received at most only the 1/3584000 of a grain, or .0000181 mg.
Four leaves were immersed as before in a solution of one part to 656,250 of water (1 gr. to 1500 oz.); but on this occasion I happened to select leaves which were very little sensitive, as on other occasions I chanced to select unusually sensitive leaves. The leaves were not more affected after 12 hrs. than [page 163] the four corresponding ones in water; but after 24 hrs. they were slightly more inflected. Such evidence, however, is not at all trustworthy.
Twelve leaves were immersed, each in thirty minims of a solution of one part to 1,312,500 of water (1 gr. to 3000 oz.); so that each leaf received 1/48000 of a grain (.00135 mg.). The leaves were not in very good condition; four of them were too old and of a dark red colour; four were too pale, yet one of these latter acted well; the four others, as far as could be told by the eye, seemed in excellent condition. The result was as follows:—
(1) This was a pale leaf; after 40 m. about thirty-eight tentacles inflected; after 3 hrs. 30 m. the blade and many of the outer tentacles inflected; after 10 hrs. 15 m. all the tentacles but seventeen inflected, and the blade quite doubled up; after 24 hrs. all the tentacles but ten more or less inflected. Most of them were closely inflected, but twenty-five were only sub-inflected.
(2) After 1 hr. 40 m. twenty-five tentacles inflected; after 6 hrs. all but twenty-one inflected; after 10 hrs. all but sixteen more or less inflected; after 24 hrs. re-expanded.