plant with 9 leaves pinned out, was exposed for 35 — 40 m. to a clear sky and to nearly the same, or perhaps a rather lower, temperature (for the thermometer by an accident had been left on a [page 293] sun-dial close by), and 8 of these leaves were killed.  Of the free leaves (those on the trailing branches not being considered), a good many were killed, but their number, compared with the uninjured ones, was small.  Finally, taking the three trials together, 24 leaves, extended horizontally, were exposed to the zenith and to unobstructed radiation, and of these 20 were killed and 1 injured; whilst a relatively very small proportion of the leaves, which had been allowed to go to sleep with their leaflets vertically dependent, were killed or injured.

The cotyledons of several plants were prepared for trial, but the weather was mild and we succeeded only in a single instance in having seedlings of the proper age on nights which were clear and cold.  The cotyledons of 6 seedlings of Mimosa pudica were fastened open on cork and were thus exposed for 1 h. 45 m. to a clear sky, with the temperature on the surrounding ground at 29o F.; of these, 3 were killed.  Two other seedlings, after their cotyledons had risen up and had closed together, were bent over and fastened so that they stood horizontally, with the lower surface of one cotyledon fully exposed to the zenith, and both were killed.  Therefore of the 8 seedlings thus tried 5, or more than half, were killed.  Seven other seedlings with their cotyledons in their normal nocturnal position, viz., vertical and closed, were exposed at the same time, and of these only 2 were killed.* Hence it appears, as far as these few trials tell anything, that the vertical position at night of the cotyledons of Mimosa pudica protects them to a certain degree from the evil effects of radiation and cold.]

Concluding Remarks on the Radiation from Leaves at Night.—­We exposed on two occasions during the summer to a clear sky several pinned-open leaflets of Trifolium pratense, which naturally rise at night, and of Oxalis purpurea, which naturally sink at night (the plants growing out of doors), and looked at

* We were surprised that young seedlings of so tropical a plant as Mimosa pudica were able to resist, as well as they did, exposure for 1 hr. 45 m. to a clear sky, the temperature on the surrounding ground being 29o F. It may be added that seedlings of the Indian ‘Cassia pubescens’ were exposed for 1 h. 30 m. to a clear sky, with the temp. on the surrounding ground at -2o C., and they were not in the least injured. [page 294]

them early on several successive mornings, after they had assumed their diurnal positions.  The difference in the amount of dew on the pinned-open leaflets and on those which had gone to sleep was generally conspicuous; the latter being sometimes absolutely dry, whilst the leaflets which had been horizontal were coated with large beads of dew.  This shows how much cooler the leaflets fully exposed to the zenith must have become, than those which stood almost vertically, either upwards or downwards, during the night.