poor in nitrogen, to obtain nitrogen from some source
other than the soil in which they grow. A pea
plant in soil that contains no nitrogen products and
watered with water that contains no nitrogen, will,
after sprouting and growing for a length of time,
be found to have accumulated a considerable quantity
of fixed nitrogen in its tissues The only source of
this nitrogen has been evidently from the air which
bathes the leaves of the plant or permeates the soil
and bathes its roots This fact was at first disputed,
but subsequently demonstrated to be true, and was
found later to be associated with the combined action
of these legumes and certain soil bacteria. When
a legume thus gains nitrogen from the air, it develops
upon its roots little bunches known as root nodules
or root tubercles. The nodules are sometimes
the size of the head of a pm, and sometimes much larger
than this, occasionally reaching the size of a large
pea, or even larger. Upon microscopic examination
they are found to be little nests of bacteria In some
way the soil organisms (Fig 27) make their way into
the roots of the sprouting plant, and finding there
congenial environment, develop in considerable quantities
and produce root tubercles in the root. Now,
by some entirely unknown process, the legume and the
bacteria growing together succeed in extracting the
nitrogen from the atmosphere which permeates the soil,
and fixing this nitrogen in the tubercles and the
roots in the form of nitrogen compounds. The
result is that, after a proper period of growth, the
amount of fixed nitrogen in the plant is found to have
very decidedly increased (Fig 25 E).
This, of course, furnishes a starting point for the
reclaiming of the lost atmospheric nitrogen.
The legume continues to live its usual life, perhaps
increasing the store of nitrogen in its roots and
stems and leaves during the whole of its normal growth.
Subsequently, after having finished its ordinary life,
the plant will die, and then the roots and stems and
leaves, falling upon the ground and becoming buried,
will be seized upon by the decomposition bacteria
already mentioned. The nitrogen which has thus
become fixed in their tissues will undergo the destructive
changes already described. This will result eventually
in the production of nitrates. Thus some of the
lost nitrogen is restored again to the soil in the
form of nitrates, and may now start on its route once
more around the cycle of food.
It will be seen, then, that the food cycle is a complete
one. Beginning with the mineral ingredients in
the soil, the food matter may start on its circulation
from the soil to the plant, from the plant to the
animal, from the animal to the bacterium and from
the bacterium through a series of other bacteria back
again to the soil in the condition in which it started.
If, perchance, in this progress around the circle
some of the nitrogen is thrown off at a tangent, this,
too, is brought back again to the circle through the