Crossed seeds from the crossed plants, and self-fertilised seeds from the self-fertilised plants of the last generation, were sown thickly on opposite sides of a small pot, Number 1. The two tallest plants on each side were measured after they had flowered, and the two crossed ones were 12 and 7 1/2 inches, and the two self-fertilised ones 8 and 5 1/2 inches in height; that is, in the ratio of 100 to 69. Twenty flowers on the crossed plants were again crossed and produced twenty capsules; ten of which contained 1.33 grain weight of seeds. Thirty flowers on the self-fertilised plants were again self-fertilised and produced twenty-six capsules; ten of the best of which (many being very poor) contained only .87 grain weight of seeds; that is, in the ratio of 100 to 65 by weight.
The superiority of the crossed over the self-fertilised plants was proved in various ways. Self-fertilised seeds were sown on one side of a pot, and two days afterwards crossed seeds on the opposite side. The two lots of seedlings were equal until they were above half an inch high; but when fully grown the two tallest crossed plants attained a height of 12 1/2 and 8 3/4 inches, whilst the two tallest self-fertilised plants were only 8 and 5 1/2 inches high.
In a third pot, crossed seeds were sown four days after the self-fertilised, and the seedlings from the latter had at first, as might have been expected, an advantage; but when the two lots were between 5 and 6 inches in height, they were equal, and ultimately the three tallest crossed plants were 11, 10, and 8 inches, whilst the three tallest self-fertilised were 12, 8 1/2, and 7 1/2 inches in height. So that there was not much difference between them, the crossed plants having an average advantage of only the third of an inch. The plants were cut down, and without being disturbed were transplanted into a larger pot. Thus the two lots started fair on the following spring, and now the crossed plants showed their inherent superiority, for the two tallest were 13 inches, whilst the two tallest self-fertilised plants were only 11 and 8 1/2 inches in height; or as 100 to 75. The two lots were allowed to fertilise themselves spontaneously: the crossed plants produced a large number of capsules, whilst the self-fertilised produced very few and poor ones. The seeds from eight of the capsules on the crossed plants weighed .65 grain, whilst those from eight of the capsules on the self-fertilised plants weighed only .22 grain; or as 100 to 34.
The crossed plants in the above three pots, as in almost all the previous experiments, flowered before the self-fertilised. This occurred even in the third pot in which the crossed seeds were sown four days after the self-fertilised seeds.
Lastly, seeds of both lots were sown on opposite sides of a large pot in which a Fuchsia had long been growing, so that the earth was full of roots. Both lots grew miserably; but the crossed seedlings had an advantage at all times, and ultimately attained to a height of 3 1/2 inches, whilst the self-fertilised seedlings never exceeded 1 inch. The several foregoing experiments prove in a decisive manner the superiority in constitutional vigour of the crossed over the self-fertilised plants.