Twelve flowers on the crossed plants of the last generation growing in the four large pots just mentioned, were crossed with pollen from a crossed plant growing in one of the other pots; and twelve flowers on the self-fertilised plants were fertilised with their own pollen.  All these flowers of both lots produced fine capsules.  Ten of the crossed capsules contained by weight 38.92 grains of seeds, and ten of the self-fertilised capsules 37.74 grains; or as 100 to 97.  Some of these seeds in an equal state of germination were planted in pairs on the opposite sides of five large pots.  A good many of the crossed seeds germinated before the self-fertilised, and were of course rejected.  The plants thus raised were measured when several of them were in full flower.

Table 6/85.  Nicotiana tabacum (second generation).

Heights of plants measured in inches.

Column 1:  Number (Name) of Pot.

Column 2:  Crossed Plants.

Column 3:  Self-fertilised Plants.

Pot 1 :  14 4/8 :  27 6/8. 
Pot 1 :  78 4/8 :  8 6/8. 
Pot 1 :  9 :  56.

Pot 2 :  60 4/8 :  16 6/8. 
Pot 2 :  44 6/8 :  7. 
Pot 2 :  10 :  50 4/8.

Pot 3 :  57 1/8 :  87 (A). 
Pot 3 :  1 2/8 :  81 2/8 (B).

Pot 4 :  6 6/8 :  19. 
Pot 4 :  31 :  43 2/8. 
Pot 4 :  69 4/8 :  4.

Pot 5 :  99 4/8 :  9 4/8. 
Pot 5 :  29 2/8 :  3.

Total :  511.63 :  413.75.

The thirteen crossed plants here average 39.35, and the thirteen self-fertilised plants 31.82 inches in height; or as 100 to 81.  But it would be a very much fairer plan to exclude all the starved plants of only 10 inches and under in height; and in this case the nine remaining crossed plants average 53.84, and the seven remaining self-fertilised plants 51.78 inches in height, or as 100 to 96; and this difference is so small that the crossed and self-fertilised plants may be considered as of equal heights.

In addition to these plants, three crossed plants were planted separately in three large pots, and three self-fertilised plants in three other large pots, so that they were not exposed to any competition; and now the self-fertilised plants exceeded the crossed in height by a little, for the three crossed averaged 55.91, and the three self-fertilised 59.16 inches; or as 100 to 106.

Crossed and self-fertilised plants of the third generation.

Table 6/86.  Nicotiana tabacum (third generation).  Seedlings from the self-fertilised plant A in pot 3, Table 6/85, of the last or second generation.

Heights of plants measured in inches.

Column 1:  Number (Name) of Pot.

Column 2:  From Self-fertilised Plant, crossed by a Crossed Plant.

Column 3:  From Self-fertilised Plant again self-fertilised, forming the third Self-fertilised generation.