I was so much surprised at this great superiority of the self-fertilised over the crossed plants, that I determined to try how they would behave in one of the pots during a second growth.  The two plants, therefore, in Pot 1 were cut down, and repotted without being disturbed in a much larger pot.  In the following year the self-fertilised plant showed even a greater superiority than before; for the two tallest flower-stems produced by the one crossed plant were only 29 4/8 and 30 1/8 inches in height, whereas the two tallest stems on the one self-fertilised plant were 49 4/8 and 49 6/8 inches; and this gives a ratio of 100 to 167.  Considering all the evidence, there can be no doubt that these self-fertilised plants had a great superiority over the crossed plants.

Crossed and self-fertilised plants of the second generation.

Table 5/71.  Lobelia fulgens (Second Generation).

Heights of flower-stems measured in inches.

Column 1:  Number (Name) of Pot.

Column 2:  Crossed Plants.

Column 3:  Self-fertilised Plants.

Pot 1 :  27 3/8 :  32 3/8. 
Pot 1 :  26 :  26 3/8. 
Pot 1 :  24 3/8 :  25 1/8. 
Pot 1 :  24 4/8 :  26 2/8.

Pot 2 :  34 :  36 2/8. 
Pot 2 :  26 6/8 :  28 6/8. 
Pot 2 :  25 1/8 :  30 1/8. 
Pot 2 :  26 :  32 2/8.

Pot 3 :  40 4/8 :  30 4/8. 
Pot 3 :  37 5/8 :  28 2/8. 
Pot 3 :  32 1/8 :  23.

Pot 4 :  34 5/8 :  29 4/8. 
Pot 4 :  32 2/8 :  28 3/8. 
Pot 4 :  29 3/8 :  26. 
Pot 4 :  27 1/8 :  25 2/8.

Pot 5 :  28 1/8 :  29. 
Pot 5 :  27 :  24 6/8. 
Pot 5 :  25 3/8 :  23 2/8. 
Pot 5 :  24 3/8 :  24.

Pot 6 :  33 5/8 :  44 2/8. 
Pot 6 :  32 :  37 6/8. 
Pot 6 :  26 1/8 :  37. 
Pot 6 :  25 :  35.

Pot 7 :  30 6/8 :  27 2/8. 
Pot 7 :  30 3/8 :  19 2/8. 
Pot 7 :  29 2/8 :  21.

Pot 8 :  39 3/8 :  23 1/8. 
Pot 8 :  37 2/8 :  23 4/8. 
Pot 8 :  36 :  25 4/8. 
Pot 8 :  36 :  25 1/8.

Pot 9 :  33 3/8 :  19 3/8. 
Pot 9 :  25 :  16 3/8. 
Pot 9 :  25 3/8 :  19. 
Pot 9 :  21 7/8 :  18 6/8.

Total :  1014.00 :  921.63.

I determined on this occasion to avoid the error of using pollen of not quite equal maturity for crossing and self-fertilisation; so that I squeezed pollen out of the conjoined anthers of young flowers for both operations.  Several flowers on the crossed plant in Pot 1 in Table 5/70 were again crossed with pollen from a distinct plant.  Several other flowers on the self-fertilised plant in the same pot were again self-fertilised with pollen from the anthers of other flowers on the same plant.  Therefore