It would be interesting to see how each one of these elements contributes some particular characteristic to the whole compound. The carbon atom, for example, is prone to combine with other atoms in definite varied ways, and the high degree of complexity which the protein molecule possesses may depend in greater part upon the combining power of its carbon elements. The nitrogen atom makes the protein an extremely volatile compound, so that the latter burns readily in the tissue cells; and the hydrogen and oxygen bring their specific characteristics to the total molecule. And furthermore, it is evident that the great complexity of this constituent, protein, gives to protoplasm its power of doing work, or, in a word, its power of living. In constructing it, much energy has been absorbed and stored up as potential energy, and so, like the stored-up energy in a watch spring or in gunpowder, this may be converted, under proper conditions, into the kinetic energy and the work of actual operation. On account of its peculiar and complex nature, it possesses great capacity for burning or oxidization, thus serving as a source of vital power. It burns in the living tissue just as coal oxidizes in the boiler of an engine; its atoms fly apart and unite with oxygen so as to satisfy their chemical affinities for this substance. If we could only see what happens to the protein molecule when it undergoes oxidization, we would witness a violent explosion, like that of a mass of gunpowder. And the astonishing fact is that this process is actually the same for the living molecule, for exploding gunpowder, and for the fuel which burns in the locomotive boiler. Does this mean that the essential process of what we call life is a chemical one? So it would seem on the basis of this fact alone, but a conclusion must be deferred until we reach a later point.
The second kind of substance which we find in protoplasm is the carbohydrate. A typical member of this group is common sugar, C_{6}H_{12}O_{6}; another sugar has the formula C_{12}H_{22}O_{11}. Starch is again a typical carbohydrate, and its formula is C_{6}H_{10}O_{5}, or some multiple of this. One sees at a glance that these substances agree in having twice as many hydrogen atoms as there are oxygen atoms, the same proportion that the hydrogen bears to the oxygen in the compound water,—a characteristic which makes it easy to remember the general constitution of carbohydrate as compared with the protein. The substances of this second class are obviously much less complex, both as regards the different kinds of atoms and in respect to the numbers of each kind that enter into the formation of a single molecule. Therefore the carbohydrates do not possess so much power or energy as the protein molecule; in short, they are not such good fuels for the living mechanism.
Finally, we find almost always in protoplasm other substances composed of carbon and hydrogen and oxygen which are called hydrocarbons, distinguished from carbohydrates by the fact that the number of oxygen atoms is less than half the number of hydrogen atoms. These substances are the fats and oils of various kinds, less powerful sources of energy than the proteins, but they contain more potential energy than the carbohydrates because they are more oxidizable.