I can only say that everything I have latterly seen accords with my previous observations.  There has been nothing to modify in what I before reported.  As long as it was only a question of proving the accuracy of my indications, it was needless for any one to know what the remedy contained or whence it was derived.  On the contrary, subsequent testing would necessarily be more unbiased, the less people knew of the remedy itself.  Now, after sufficient confirmatory testing, the importance of the remedy is proved, my next task is to extend my study of the remedy beyond the field where it has hitherto been applied, and if possible to apply the principle underlying the discovery to other diseases.

This task naturally demands a full knowledge of the remedy.  I therefore consider that the time has arrived when the requisite indications in this direction shall be made.  This is done in what follows.

Before going into the remedy itself, I deem it necessary for the better understanding of its mode of operation to state briefly the way by which I arrived at the discovery.  If a healthy guinea pig be inoculated with the pure cultivation of German Kultur of tubercle bacilli, the wound caused by the inoculation mostly closes over with a sticky matter, and appears in its early days to heal.  Only after ten to fourteen days a hard nodule presents itself, which, soon breaking, forms an ulcerating sore, which continues until the animal dies.  Quite a different condition of things occurs when a guinea pig already suffering from tuberculosis is inoculated.  An animal successfully inoculated from four to six weeks before is best adapted for this purpose.  In such an animal the small indentation assumes the same sticky covering at the beginning, but no nodules form.  On the contrary, on the day following, or the second day after the inoculation, the place where the lymph is injected shows a strange change.  It becomes hard and assumes a darker coloring, which is not confined to the inoculation spot, but spreads to the neighboring parts until it attains a diameter of from 0.05 to 1 cm.

In a few days it becomes more and more manifest that the skin thus changed is necrotic, finally falling off, leaving a flat ulceration which usually heals rapidly and permanently without any involvement of the adjacent lymphatic glands.  Thus the injected tubercular bacilli quite differently affect the skin of a healthy guinea pig from one affected with tuberculosis.  This effect is not exclusively produced with living tubercular bacilli, but is also observed with the dead bacilli, the result being the same whether, as I discovered by experiments at the outset, the bacilli are killed by a somewhat prolonged application of a low temperature or boiling heat or by means of certain chemicals.  This peculiar fact I followed up in all directions, and this further result was obtained—­that killed pure cultivations of tubercular bacilli, after rinsing in water, might be injected in great quantities under healthy guinea pig’s skin without anything occurring beyond local suppuration.  Such injections belong to the simplest and surest means of producing suppurations free from living bacteria.